Gamma-interferon investigation in rat periodontal tissue under induced dental movement

Daniel Mascarenhas da SILVEIRA, Fernanda Catharino Menezes FRANCO, Ivana Lúcia de Oliveira NASCIMENTO, Milene de Freitas Lima SALOMÃO, Telma Martins de ARAUJO

Introduction: During the orthodontic treatment, an infl ammatory process is induced, triggering series of biochemical events that results in dental movement. Stimuli such as hypoxia and mechanical deformation are the main factors responsible for the breaking of cell homeostasis, resulting in stress and liberation of several important mediators for tooth movement. To occur osseous remodeling during orthodontic treatments, regulating factors such as sub products of arachidonic acid and cytokines are released. To interferon-gamma (INF-γ), a main cytokine released after induction of adaptive immune response, is attributed the function of attracting the macrophages, which aids in removing cell rests and promote healing and reorganization of the infl amed areas. Aim: Given that some biological aspects that permeate dental movement are not yet fully clear, we aimed in this study to investigate INF-γ expression in the periodontium of rats under orthodontic movement. Methods: The sample was composed of 9 rats, whose fi rst upper right molars were moved with a force of 0,5N, for 3, 7 and 14 days. The left molars were used as controls. Results: Through immunohistochemistry, we verifi ed the absence of INF-γ expression in almost all the studied tissues, in both the traction and pressure sides.

Keywords: Interferon-gamma. Tooth movement. Immunohistochemistry.

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