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Influence of contact with blood and saline on solubility, pH and chemical composition of MTA

Lyz Cristina Furquim Canali, Carlos Alberto Herrero De Morais, Bruno Cavalini Cavenago, Rodrigo Ricci Vivan, Marco Antonio Hungaro Duarte

Objective: The aim of this study was to evaluated the solubility, pH level and chemical constitution of white MTA in contact with blood and saline solution. Methods: Thirty acrylic teeth replicas of a maxillary central incisor, with 3 mm root-end cavity were filled with white MTA. The samples were inserted individually in Eppendorf microtubes and divided into 3 groups (n = 10). In the Group 1 the specimens were not exposed to liquids (control group). In the Group 2 microtubes received 1 mL of saline solution and Group 3 received 1 mL of fresh human blood. The specimens were stored at 37 °C for 24 hours. The solubility was volumetric evaluated so the specimens were scanned by a Micro-CT and individually immersed in 10 mL of ultrapure water, after 168 hours new scans were performed. The pH level was measured after 168 hours. Sections of 2 mm thickness were analyzed using energy dispersive spectroscopy to evaluate the ionic composition. The data were statistically analyzed by the ANOVA and Tukey tests. Results: The solubility was similar (p > 0.05) for all groups. After 168 hours the pH was significantly higher for Group 2 in comparison with Group 3 (p < 0.05). In the ionic composition was found higher concentration of calcium and the contact with blood did not interfere on ion calcium release. Conclusion: The contact of MTA with blood and saline solution did not influence in the solubility, both favored an alkaline pH with increased of ion calcium release with small variations in the ionic composition.

Keywords: Endodontics; Dental materials. Materials testing. X-ray microtomography. Scanning electron microscopy

How to cite: Canali LCF, Morais CAH, Cavenago BC, Vivan RR, Duarte MAH. Influence of contact with blood and saline on solubility, pH and chemical composition of MTA. Dental Press Endod. 2016 Sept-Dec;6(3):41-5. DOI: http://dx.doi.org/10.14436/2358-2545.6.3.041-045.oar

Friday, September 22, 2017 17:41