Effect of alcohol intake on submandibular salivary glands

Tatiana Wannmacher Lepper, Denise Bertin Rojas, Virginia Cielo Rech, Luciane Rosa Feksa, Felipe Nor, Anna Christina Medeiros Fossati, João Carlos Birnfeld Wagner, Clóvis Milton Duval Wannmacher

Introduction: Chronic consumption of alcohol affects the oral mucosa and its annexes. Changes in the function of enzymes found in that tissue can change the present saliva. Objective: Thus, the objective of the present study was to investigate the effect of chronic ethanol intake on glandular regeneration and activity of the enzymes creatine kinase (CK), pyruvate kinase (PK), and acid phosphatase (AP), in the submandibular glands of Wistar rats. Materials and Methods: The test group was submitted to chronic ingestion of 40º GL ethyl alcohol for 45 days, and the control was given water. Then the animals were anesthetized with ketamine and subjected to partial excision of submandibular gland left lobe. After the regeneration period, the animals were sacrificed and the left and right submandibular glands were removed for dosage of CK, PK and AP enzymes activity and determination of proteins. Results: It was found that chronic ethanol intake increased the activity of these enzymes in the regeneration period. Conclusion: These results suggest that the regeneration process of a gland stimulate the activities of the studied enzymes in both glands, probably by a compensation mechanism and functional balance. In addition, this enzymatic activity stimulus becomes more pronounced when the regeneration occurs in the presence of ethanol, possibly in response to its toxic effect.

Keywords: Creatine kinase. Submandibular gland. Ethanol.

How to cite: Lepper TW, Rojas DB, Rech VC, Feksa LR, Nor F, Fossati ACM, Wagner JCB, Wannmacher CMD. Effect of alcohol intake on submandibular salivary glands. J Braz Coll Oral Maxillofac Surg. 2018 jan-abr;4(1):15-21. DOI: https://doi.org/10.14436/2358-2782.4.1.015-021.oar

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