Microbiological and antimicrobial evaluation of Bio-Oss®: an in vitro study

GRAZIELLA PELEGRINI, LINA NAOMI HASHIZUME, MARCUS VINICIUS REIS SÓ, ANGELO MENUCI NETO, FERNANDO BRANCO BARLETTA

Introduction: Bone substitutes are materials designed to resemble and replace human bone. Objective: The ob- jective of the study was to evaluate the contamination of the commercial product Bio-Oss® along the time after opening. Methods: Twelve vials of Bio-Oss® were tested at different time points: immediately after opening, 24 h, 48h, 72h, 7d, 14d and 21 days after. Suspensions of the commercial product were plated in culture media to evaluate yeasts, Streptococcus and total oral micro-organisms growth. The plates were incubated at 37°C for 48 hours and the number of microorganisms was determined. Antimicrobial effect of the product was also verified by a disc-diffusion test. Results: No growth of total oral microorganisms, Streptococcus or yeasts was observed along the observed time (p>0.99). The product was not presented inhibition of total oral microorganisms in disc-diffusion test (p=0.1). Conclusion: It can be concluded that Bio-Oss® does not present microbial contamination in an interval of up to 21 days, when manipulated and stored aseptically.

Keywords: Apicoectomy. Biological contamination. Bone regeneration. Microbiological techniques.

How to cite: Pelegrini G, Hashizume LN, Só MVR, Menuci Neto A, Barletta FB. Microbiological and antimicrobial evaluation of Bio-Oss®: an in vitro study. J Braz Coll Oral Maxillofac Surg. 2021 May-Aug;7(2):22-8.

Tuesday, May 14, 2024 13:17